Nuclear Transfer into Mouse Oocytes
نویسندگان
چکیده
منابع مشابه
Establishment of mouse embryonic stem cell lines from somatic cell nuclei by nuclear transfer into aged, fertilization-failure mouse oocytes
It has been widely assumed that fresh oocytes are required for the establishment of embryonic stem cells (ES cells) via somatic cell nuclear transfer (SCNT). Such cell lines are referred to as ‘NT-ES cells’ [1]. This presumed requirement, however, gives rise to fundamental ethical concerns surrounding its potential application to human cells, as fresh oocytes must be obtained from a healthy fem...
متن کاملNuclear transfer to eggs and oocytes.
We review experiments in which somatic cell nuclei are transplanted singly to enucleated eggs (metaphase II) in amphibia and mammals and as multiple nuclei to the germinal vesicle of amphibian oocytes (prophase I). These experiments have shown the totipotency of some somatic cell nuclei, as well as switches in cell type and changes in gene expression. Abnormalities of nuclear transplant embryo ...
متن کاملSomatic and embryonic cell nucleus transfer into intact and enucleated immature mouse oocytes.
BACKGROUND The aim of our study was to evaluate the possibility of embryonic or somatic cell haploidization after fusion with intact or enucleated immature oocytes which were subsequently cultured in vitro. Embryonic or somatic cell nuclei do not undergo premature chromosome condensation when fused to intact or enucleated immature oocytes whose maturation is prevented by dibutyryl cyclic AMP (d...
متن کاملGerminal vesicle transfer between fresh and cryopreserved immature mouse oocytes.
BACKGROUND We assessed the maturational competence and the chromosomal pattern of mouse oocytes reconstructed by germinal vesicle (GV) transfer technique using nuclear and/or cytoplasmic components from cryopreserved GV stage oocytes. METHODS From 657 GV oocytes (326 fresh and 331 frozen/thawed), four groups of reconstructed oocytes were obtained by micromanipulation and electrofusion: fresh ...
متن کاملAn intercellular pathway for glucose transport into mouse oocytes.
Glucose is an essential nutrient for mammalian cells. Emerging evidence suggests that glucose within the oocyte regulates meiotic maturation. However, it remains controversial as to whether, and if so how, glucose enters oocytes within cumulus-oocyte complexes (COCs). We used a fluorescent glucose derivative (6-NBDG) to trace glucose transport within live mouse COCs and employed inhibitors of g...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2006
ISSN: 1940-087X
DOI: 10.3791/116